Journal: Technology and Health Care

Article Title: Prediction and identification of epitopes in the Echinococcus multilocularis thrombospondin 3 antigen

doi: 10.3233/THC-212983

Figure Lengend Snippet: The enzyme-linked immunosorbent assay test on the cytokines of mouse spleen lymphocytes immunized with thrombospondin 3. A: The interleukin-2 test; B: the interferon-y test; C: the tumor necrosis factor- β test; D: the interleukin-4 test; E: the interleukin-17 test. P * ⁣ * * < 0.001.

Article Snippet: PE-labeled anti-mouse IL-4 flow antibody , BD, USA.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Technology and Health Care

Article Title: Prediction and identification of epitopes in the Echinococcus multilocularis thrombospondin 3 antigen

doi: 10.3233/THC-212983

Figure Lengend Snippet: The enzyme-linked immune absorbent spot test of the cytokines of mouse spleen lymphocytes immunized with thrombospondin 3. A: The interleukin-2 test; B: the interferon- γ test; C: the tumor necrosis factor- β test; D: the interleukin-4 test. P * ⁣ * * < 0.001.

Article Snippet: PE-labeled anti-mouse IL-4 flow antibody , BD, USA.

Techniques: Spot Test

Journal: Technology and Health Care

Article Title: Prediction and identification of epitopes in the Echinococcus multilocularis thrombospondin 3 antigen

doi: 10.3233/THC-212983

Figure Lengend Snippet: Experimental reagents

Article Snippet: PE-labeled anti-mouse IL-4 flow antibody , BD, USA.

Techniques: Cell Culture, Labeling, Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot

Journal: PLOS Global Public Health

Article Title: Linkage to HIV care and hypertension and diabetes control in rural South Africa: Results from the population-based Vukuzazi Study

doi: 10.1371/journal.pgph.0001221

Figure Lengend Snippet: Unweighted population prevalence of traditional risk factors among HIV negative versus HIV positive/successful ART adults with hypertension and/or diabetes mellitus in uMkhanyakude, KwaZulu-Natal, South Africa.

Article Snippet: Participants with a positive HIV immunoassay had reflex measurement of HIV-1 RNA viral load (Abbott RealTime HIV-1 Viral Load [Abbott, IL, USA]) and CD4+ cell count (BD FACS Calibur flow cytometer, BD Bioscience [San Jose, CA, USA]).

Techniques:

Journal: The Journal of Nutrition

Article Title: Impaired Hematological Status Increases the Risk of Mortality among HIV-Infected Adults Initiating Antiretroviral Therapy in Tanzania

doi: 10.1093/jn/nxaa172

Figure Lengend Snippet: Baseline characteristics, hematological status, and iron biomarkers among a randomly selected subcohort of 500 HIV-infected adults initiating antiretroviral therapy in Dar es Salaam, Tanzania, 2006–2010 1

Article Snippet: Blood specimens were collected at baseline and every 4 mo postenrollment for determination of absolute CD4 T-cell count (FACSCalibur flow cytometer, Becton Dickinson) and complete blood count (AcT5 Diff AL analyzer, Beckman Coulter).

Techniques: Concentration Assay

Journal: BMC Health Services Research

Article Title: Field evaluation in Chad of community usage of CD4 T lymphocyte counting by alternative single-platform flow cytometry

doi: 10.1186/1472-6963-13-373

Figure Lengend Snippet: Gating strategy for the volumetric Auto40 flow cytometer (Apogee Flow Systems Ltd, Hemel Hempstead, UK), as depicted from typical figure provided by “Auto-Lymphocyte” software (Apogee Flow Systems Ltd) for the counting of CD4 T lymphocytes in one K 3 -EDTA-anticoagulated blood sample, incubated with phycoerythrin (PE)-conjugated anti-CD4 and PE-Dyomics649-conjugated anti-CD45 monoclonal antibodies (Apogee Flow Systems Ltd): (A) CD45-PE-Dyomics649 × axis-light scatter dot plot scattergram; the primary CD45 gating differentiates clearly the CD45-positive lymphocytes and monocyte populations, and the population of polymorphonuclear cells; (B) CD4-PE × axis-light scatter dot plot scattergram; double secondary CD4 gating allows counting independently the population of CD4-negative lymphocyte and CD4-positive lymphocytes; the CD4 T lymphocytes count in absolute number is given by the CD4-positive gating; the CD4 T lymphocytes count in percentage corresponds to the ratio of CD4-positive lymphocytes count in absolute number to the total CD4-postive and CD4-negative lymphocytes counts, corresponding to the sum of both secondary gating.

Article Snippet: In brief, direct volumetric CD4 T cell measurements are performed on the Auto40 using phycoerythrin (PE)-conjugated anti-CD4 and PE-Dyomics649-conjugated anti-CD45 monoclonal antibodies (Apogee Flow Systems Ltd).

Techniques: Flow Cytometry, Software, Incubation, Bioprocessing

Journal: BMC Health Services Research

Article Title: Field evaluation in Chad of community usage of CD4 T lymphocyte counting by alternative single-platform flow cytometry

doi: 10.1186/1472-6963-13-373

Figure Lengend Snippet: Map showing the location of the 22 officially approved health care centers devoted to follow up adult and children patients infected with HIV-1 thorough Chad in 2013 (Am Timane, Abeche, Adre, Ati, Biltine, Bol, Bongor, Doba, Fada, Faya-Largeau, Gos Beïda, Kelo, Koumra, Lere, Maïssala, Mao, Mongo, Moundou, N’Djamena, Ngouri, Pala and Sahr), as well as the 8 centers equipped by the Auto40 flow cytometers (Apogee Flow Systems Ltd) (2 in N’Djamena at the hôpital Militaire D’Instruction, and the Centre D’Appui Psycho-Médico-Social Al-Nadjma; 1 in Mongo, Bongor, Doba, Kelo, Koumra and Maïssala), and the other 9 centers equipped by FACSCount (Becton Dickinson) (2 in N’Djamena; 1 in Abeche, Adre, Gos Beïda, Moundou, Pala and Sahr), or by CyFlow (Partec) (Adre, Biltine, Maïssala, Mao and Ngouri). Note that 5 health care centers for HIV-1-infected patients (Ati; Bol; Fada; Faya-Largeau; Lere) are not yet equipped for CD4 T cell enumeration. The reference laboratory of the hôpital de la Liberté, N’Djamena, is equipped by FACSCalibur (Becton Dickinson). Note that only the health care centers involved in caring HIV-infected patients are shown in the map.

Article Snippet: In brief, direct volumetric CD4 T cell measurements are performed on the Auto40 using phycoerythrin (PE)-conjugated anti-CD4 and PE-Dyomics649-conjugated anti-CD45 monoclonal antibodies (Apogee Flow Systems Ltd).

Techniques: Infection

Journal: BMC Health Services Research

Article Title: Field evaluation in Chad of community usage of CD4 T lymphocyte counting by alternative single-platform flow cytometry

doi: 10.1186/1472-6963-13-373

Figure Lengend Snippet: Main characteristics describing the implementation and the 5 years usage (2008–2013) of 8 single-platform, volumetric, Auto40 flow cytometers for community-based point-of-care CD4 T lymphocytes enumeration installed in Chad

Article Snippet: In brief, direct volumetric CD4 T cell measurements are performed on the Auto40 using phycoerythrin (PE)-conjugated anti-CD4 and PE-Dyomics649-conjugated anti-CD45 monoclonal antibodies (Apogee Flow Systems Ltd).

Techniques: Activity Assay, Cell Counting, Control

Journal: PLoS ONE

Article Title: Reliable and Accurate CD4+ T Cell Count and Percent by the Portable Flow Cytometer CyFlow MiniPOC and “CD4 Easy Count Kit-Dry”, as Revealed by the Comparison with the Gold Standard Dual Platform Technology

doi: 10.1371/journal.pone.0116848

Figure Lengend Snippet: Correlation and R 2 values for CD4+ T cell counts obtained by Cyflow MiniPOC and CyFlow Counter by using the CD4 easy count kit (A) and the CD4% easy count kit (B).

Article Snippet: We have tested the accuracy, the precision and the carry-over contamination of Partec CyFlow MiniPOC, a portable and economically affordable flow cytometer designed for CD4+ count and percentage, used along with the “CD4% Count Kit-Dry”.

Techniques:

Journal: PLoS ONE

Article Title: Reliable and Accurate CD4+ T Cell Count and Percent by the Portable Flow Cytometer CyFlow MiniPOC and “CD4 Easy Count Kit-Dry”, as Revealed by the Comparison with the Gold Standard Dual Platform Technology

doi: 10.1371/journal.pone.0116848

Figure Lengend Snippet: Bland-Altman plot comparing absolute CD4+ T cell counts obtained by Cyflow MiniPOC and CyFlow Counter by using the CD4 easy count kit (A) and the CD4% easy count kit (B).

Article Snippet: We have tested the accuracy, the precision and the carry-over contamination of Partec CyFlow MiniPOC, a portable and economically affordable flow cytometer designed for CD4+ count and percentage, used along with the “CD4% Count Kit-Dry”.

Techniques:

Journal: PLoS ONE

Article Title: Reliable and Accurate CD4+ T Cell Count and Percent by the Portable Flow Cytometer CyFlow MiniPOC and “CD4 Easy Count Kit-Dry”, as Revealed by the Comparison with the Gold Standard Dual Platform Technology

doi: 10.1371/journal.pone.0116848

Figure Lengend Snippet: Linear Regression(A) and Bland-Altman Plot (B) for CD4+ T cell percent obtained by Cyflow MiniPOC and CyFlow Counter by using the CD4% easy count kit.

Article Snippet: We have tested the accuracy, the precision and the carry-over contamination of Partec CyFlow MiniPOC, a portable and economically affordable flow cytometer designed for CD4+ count and percentage, used along with the “CD4% Count Kit-Dry”.

Techniques:

Journal: PLoS ONE

Article Title: Reliable and Accurate CD4+ T Cell Count and Percent by the Portable Flow Cytometer CyFlow MiniPOC and “CD4 Easy Count Kit-Dry”, as Revealed by the Comparison with the Gold Standard Dual Platform Technology

doi: 10.1371/journal.pone.0116848

Figure Lengend Snippet: Correlation and R 2 values for CD4+ T cell count (A) and percentage (B) obtained by Cyflow MiniPOC and BC FC 500 reference system.

Article Snippet: We have tested the accuracy, the precision and the carry-over contamination of Partec CyFlow MiniPOC, a portable and economically affordable flow cytometer designed for CD4+ count and percentage, used along with the “CD4% Count Kit-Dry”.

Techniques: Cell Counting

Journal: PLoS ONE

Article Title: Reliable and Accurate CD4+ T Cell Count and Percent by the Portable Flow Cytometer CyFlow MiniPOC and “CD4 Easy Count Kit-Dry”, as Revealed by the Comparison with the Gold Standard Dual Platform Technology

doi: 10.1371/journal.pone.0116848

Figure Lengend Snippet: Bland-Altman plot comparing absolute CD4+ T cell count (A) and percentage (B) obtained by Cyflow MiniPOC and BC FC 500 reference system

Article Snippet: We have tested the accuracy, the precision and the carry-over contamination of Partec CyFlow MiniPOC, a portable and economically affordable flow cytometer designed for CD4+ count and percentage, used along with the “CD4% Count Kit-Dry”.

Techniques: Cell Counting

Journal: PLoS ONE

Article Title: Reliable and Accurate CD4+ T Cell Count and Percent by the Portable Flow Cytometer CyFlow MiniPOC and “CD4 Easy Count Kit-Dry”, as Revealed by the Comparison with the Gold Standard Dual Platform Technology

doi: 10.1371/journal.pone.0116848

Figure Lengend Snippet: Bar charts with error bars indicating the standard deviation of CD4 T cell count (A) and CD4% (B) of a sample analyzed 10 times in single or in bulk. CV = Coefficient of variation.

Article Snippet: We have tested the accuracy, the precision and the carry-over contamination of Partec CyFlow MiniPOC, a portable and economically affordable flow cytometer designed for CD4+ count and percentage, used along with the “CD4% Count Kit-Dry”.

Techniques: Standard Deviation, Cell Counting

Journal: PLoS ONE

Article Title: Reliable and Accurate CD4+ T Cell Count and Percent by the Portable Flow Cytometer CyFlow MiniPOC and “CD4 Easy Count Kit-Dry”, as Revealed by the Comparison with the Gold Standard Dual Platform Technology

doi: 10.1371/journal.pone.0116848

Figure Lengend Snippet: The CD4 absolute count (A) and CD4% (B) were plotted as the mean value of three analysis sequences. Error bars indicate the standard deviation of three measurements.

Article Snippet: We have tested the accuracy, the precision and the carry-over contamination of Partec CyFlow MiniPOC, a portable and economically affordable flow cytometer designed for CD4+ count and percentage, used along with the “CD4% Count Kit-Dry”.

Techniques: Standard Deviation

Journal: The Lancet Regional Health: Western Pacific

Article Title: A simple, feasible, efficient and safe treatment strategy of sofosbuvir/velpatasvir for chronic HCV/HIV-1 coinfected patients regardless of HCV genotypes: a multicenter, open-label study in China

doi: 10.1016/j.lanwpc.2023.100749

Figure Lengend Snippet: Demographic characteristics of study patients.

Article Snippet: Absolute CD4+ T lymphocyte count, CD8+ T lymphocyte count and CD4/CD8 ratio were measured using a BD CantoII flow cytometer with CD3/CD4/CD8 trichrome fluorescence reagent from BD according to the instructions.

Techniques: Transmission Assay, AST Assay